Effect of Some Commercial Antacids and Tea Drink on the In- Vitro Availability of Frusemide

The effect of ultrasound on caffeine transport through rabbitâ€TMs skin from four different gel matrices respectively made fromHPMC, methylcellulose, gelatin or carbopol 940 has been evaluated. The drug transport without ultrasound application was not detectable during the time course of the study. Ultrasound application has showed an increase drug transport (in terms of percent of drug transported, drug flux and permeability coefficient) with variable degrees according to the type of the applied gel. The order of % penetration was 25.6, 26.17, 32.72 and 65.02% w/v for, carbopol 940, gelatin, methyl cellulose and HPMC, respectively with corresponding increases in the values of flux and permeability coefficient in the same order. In vivo experiments were in agreement with the in vitro results. The amount of drug transported to the blood of living rabbits in term of peak concentration and AUC0-8 of the drug concentration plasma /time curves were significantly higher in the same order rank of the up mentioned gels. The viscosity was increased in the order of carbopol 940 < HPMC < gelatin < methylcellulose with respective values of 968.3, 1100.2, 2430 and 3352 m. Pa. Histological examination of rabbit skin has shown that no changes in the skin structure after ultrasound treatment at the same site twice daily for 3 consecutive days.